Paper
Insulin Sensitivity Enhancement of the Mixture of Tinospora Crispa and Gelam (Melaleuca Cajuputi) Honey and Its Antiproliferative Activity on Hepatocellular Carcinoma, HepG2: A Preliminary Study
- Authors:
- Hamzah Fansuri Hassan; Rosmadi Mohd Yusof; Nabilatul Hani Mohd Radzman; Muhammad Ashraf Mohd Salleh; Wan Iryani Wan Ismail; Mohd Nazri Abu
- Abstract
- This study aims to investigate the effect of Tinosporacrispa and Gelam (Melaleucacajupati) honey mixture on human hepatocellular carcinoma (HepG2) and normal human hepatocytes(WRL-68) cell lines. Cell cytotoxicity was measured using MTS assay. The IC50obtained was 42.67% in HepG2 and none in the WRL-68. Western blotting showed an increment in phosphorylation of tyrosine (PY20), indicating induction of Caspase 3 apoptotic proteins, whereas, the gene product of insulin growth-like receptor (IGF-1R) was reduced, indicating cancer cell inhibition. It is concluded that the elevation of Caspase 3 and the inhibition of IGF-1R indicates the ability of this mixture to simultaneously promote apoptosis and sensitized insulin in HepG2 cells.
- Keywords
- Tinosporacrispa; Gelam Honey; Apoptosis; Cytotoxicity; Insulin Sensitivity; Hepg2; WRL-68
- StartPage
- 48
- EndPage
- 54
- Doi