Paper
Effect of Plant Growth Regulators on Direct Shoot Regeneration of Wheat Immature Embryonic Explants
- Authors:
- Arash Mokhtari; B. Yazdi Samadi; M. Omidi; M. Otroshy; Z. Moeini; H. Alizadeh
- Abstract
- Short-term high efficiency protocol for direct shoot regeneration (DSR) and induce of multiple shoot regeneration (MSR) for in vitro plant genetic transformation or propagation was developed for wheat. This tissue culture and regeneration system with no callus formation, takes about 2 months to finish, shorter than any of the available systems requiring 3–4 months. Cutted shoot embryonic meristem (CSEM), shoot embryonic meristem with the scutellum (SEMS), shoot embryonic meristem (SEM) and scutellum (S), were excised from immature embryos and cultured in DSR medium. Rapid inductions of direct shoot buds and multiple shoot regeneration (MSR) in some of explants roots were got. Then, plants were transferred to the soil and showed normal growth and fertility in comparison with the seed-grown plants. The effects of different explants, light conditions and various combinations of plant growth regulators on direct shoot regeneration of wheat were investigated. The best response for multiple shoot regeneration from (SEMS) explants was observed on modified MS medium containing 2 mgl-1 2, 4-D and 10 mgl-1 BAP. Also 4-week period of exposure to darkness followed by light exposure was important for shoot regeneration. The average number of multiplied shoots per explants and explants with multiple shoots per culture dish was 8.38 and 5.46 among the cultivars after 8-9 weeks, respectively.
- Keywords
- Wheat; Direct Shoot Regeneration; Immature Explants; 2,4-D
- StartPage
- 74
- EndPage
- 80
- Doi
- 10.18005/JAEB0103004