Paper
Immunohistochemical Analysis of WT1, EGFR, E-cadherin, beta-catenin and p53 in 43 Moroccan Epithelial Ovarian Tumours
- Authors:
- Mariam Amrani; Lorenzo Memeo; Habiba Kadiri; Hind Charhi; Mohamed Alaoui Belabbas; Mahesh M. Mansukhani
- Abstract
- Aim of the study: Immunohistochemical evaluation of WT1, E-cadherin, beta-catenin, EGFR and p53 on Tissue MicroArray (TMA) of 43 Moroccan benign, borderline and invasive epithelial ovarian tumours. Materials and methods: All 43 cases were collected from the pathology department of the Institut National d’Oncologie in Rabat, Morocco, and comprised 34 carcinomas, 4 borderline serous and mucinous tumours and 5 benign tumours. Patients were between 20 and 74 years old with a mean age of 50 years. TMAs and the IHC study were supported by a grant from the IAAE (International Agency of Atomic Energy) and prepared in the pathology department of Columbia University in New York. 3 cores were selected from each case, and the peroxydase-anti peroxydase technique was used for the study of the different markers (DAKO Cytovision, Carpinteria CA). Results: 23.25% of the cases (10/43) were WT1 positive and were serous tumours (including one poorly differentiated adenocarcinoma). 72% of the cases (31/43) showed reduced (19/43) or no (12/43) membranous expression of E-cadherin, and all the tumours showed reduced membranous expression with cytoplasmic expression (5/43) or no expression (38/43) of beta-catenin. p53 overexpression (13/43) was exclusively observed in 58% (11/19) of the serous carcinomas and 2/3 poorly to moderately differentiated adenocarcinomas, of which 9/13 were EGFR + and 6/13 were E-cadherin +. 70% of the cases (30/43) showed EGFR membrane staining, and 2 cases were not interpretable. Conclusion: TMA is a feasible tool to study a large number of cases allowing comparative analysis of the expression of different biomarkers. To our knowledge, this is the first study of 5 biomarkers to be done on TMAs from 43 moroccan benign, borderline and invasive epithelial ovarian tumour samples. This would allow for larger studies with the aim of analyzing the significance of these biological markers and their impact in clinical trials.
- Keywords
- Ovarian Epithelial Tumours; Tissue Microarray; EGFR Protein Marker; Suppressor Protein Markers; Wnt Protein Markers
- StartPage
- 11
- EndPage
- 17
- Doi
- 10.5963/BER0301002