Paper

Ortho-Hydroxy Derivatives of 2,5-Diaryl-1,3-Oxazole as Fluorescent Probes to Monitor the Changes in Human Erythrocyte Membranes under the Influence of Low Molecular Weight Cryoprotectants


Authors:
Yevgen Korniyenko; Yevgen Posokhov
Abstract
It has been shown that derivatives of 2,5-diaryl-1,3-oxazole can be used as fluorescent probes to monitor the changes in physico-chemical properties of human erythrocyte membranes under the action of low molecular weight cryoprotectants such as glycerol and 1,2-propanediol. It has been found that increase in cryoprotectant concentration increases hydration of erythrocyte membrane. The minimal concentration of the cryoprotectant, which causes the perturbation of the erythrocyte membrane, has been estimated to be 0.5 % and 5.0 % for glycerol and 1,2-propanediol, correspondingly. Using a set of fluorophores with different locations in lipid bilayer enabled us to show that the increase in the membrane hydration occurs in polar regions of the membrane (in the areas of glycerol residues of phospholipids, carbonyl groups of phospholipids and in the area of methylene groups in the vicinity of carbonyl groups of phospholipids), while no changes in hydration have been observed in the most hydrophobic regions of the lipid bilayer: in the area of the methylene groups of phospholipids near the centre of lipid bilayer and in the center of lipid bilayer. The suggested fluorescent probes can be used to monitor the changes of physico-chemical properties in different regions of lipid bilayer under the action of the cryoprotectants and to determine optimal concentrations of cryoprotectants for the low-temperature storage of cells.
Keywords
Fluorescent Probe; Human Erythrocyte Membrane; Cryoprotectants; Glycerol; 1,2-Propanediol
StartPage
32
EndPage
36
Doi
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