Paper
Fingerprinting of Cotton (Gossypium Spp.) Hybrids and their Parental Lines Using Microsatellite Markers and their Utilization in Genetic Purity Assessment
- Authors:
- Rao P S; Aruna P; Anuradha G; Keshavulu K
- Abstract
- Microsatellite markers are used for fingerprinting of cotton hybrids in order to assess variation within parental lines. Sixteen microsatellite markers were employed to fingerprint to six hybrids and their parental lines. Five SSR markers were found to be polymorphic across the hybrids and to produce unique fingerprints for each of the six hybrids. These highly informative primers not only differentiated the parent genotypes, but also confirmed the parentage of the true hybrids. The microsatellite marker, BNL 3449 amplified alleles specific to different parental lines of NSPHH 5; likewise, BNL 3255 primer for NDLHH 240 and WGHH 2, JESPR 148 primer for LAHH 4, BNL1317 for CSH 198 and BNL 3090 for CICR 2. Results indicate that SSR procedures are excellent genomic tools for parentage confirmation and hybridity determination, and may also enhance efficiency of breeding programmes.
- Keywords
- Hybrid Cotton; Ssrs; DNA Fingerprinting; Genetic Purity
- StartPage
- 120
- EndPage
- 124
- Doi
- 10.18005/JAEB0303005