Journal of Medical Research and Development          
Journal of Medical Research and Development(JMRD)
Frequency: Quarterly
Insulin Sensitivity Enhancement of the Mixture of Tinospora Crispa and Gelam (Melaleuca Cajuputi) Honey and Its Antiproliferative Activity on Hepatocellular Carcinoma, HepG2: A Preliminary Study
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This study aims to investigate the effect of Tinosporacrispa and Gelam (Melaleucacajupati) honey mixture on human hepatocellular carcinoma (HepG2) and normal human hepatocytes(WRL-68) cell lines. Cell cytotoxicity was measured using MTS assay. The IC50obtained was 42.67% in HepG2 and none in the WRL-68. Western blotting showed an increment in phosphorylation of tyrosine (PY20), indicating induction of Caspase 3 apoptotic proteins, whereas, the gene product of insulin growth-like receptor (IGF-1R) was reduced, indicating cancer cell inhibition. It is concluded that the elevation of Caspase 3 and the inhibition of IGF-1R indicates the ability of this mixture to simultaneously promote apoptosis and sensitized insulin in HepG2 cells.
Keywords:Tinosporacrispa; Gelam Honey; Apoptosis; Cytotoxicity; Insulin Sensitivity; Hepg2; WRL-68
Author: Wan Iryani Wan Ismail1, Mohd Nazri Abu2, Muhammad Ashraf Mohd Salleh1, Nabilatul Hani Mohd Radzman1, Rosmadi Mohd Yusof1, Hamzah Fansuri Hassan2
1.Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia
2.Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia
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