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Effects of Biofield Therapy on Calcium Release in Immortalized Mouse Keratinocyte HaCaT Cells
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Abstract:
Objective: Observe the response on the release of calcium into mouse keratinocyte HaCaT cells subjected to biofield therapy (BT) through a pranic healing technique. Design: This was a pilot experimental study. Settings/location: The study was conducted in a laboratory at Simon Bolivar University. Subjects: Mouse keratinocyte HaCaT cells. Interventions: The intervention consisted of a 15-minute biofield therapy using a pranic healing technique. Outcome measures: Cells were loaded with 5 μM calcium indicator Fura 2-AM to monitor changes in intracellular calcium concentration. Cell population was separated into two groups: a control group where cells received no stimulation and the other experimental group where pranic healing was applied. Results: The cells that were treated with pranic healing showed a significant increase in intracellular calcium concentration as compared with untreated cells. Such increase in calcium concentration is consistent with the depletion of intracellular stores. By the action of Thapsigargin (TG) peak, calcium release is equivalent to cells exposed to pranic healing in comparison to control cells. One possible explanation for this observed result is that store of intracellular calcium had been stimulated by pranic healing and hence the resulting drain is lower. These results allow us to infer that pranic healing has an action on intracellular calcium storage but does not allow us to clarify how calcium has been stimulated.
Keywords:Fura-2 AM; Mouse Keratinocyte HaCaT Cells; [Ca]i; Issuing Biofield Therapy (BT); Pranic Healing Technique; Information Theory; Biofield
Author: Silva Ricardo1, Herrera Alvaro2, Velásquez Hordep2
1.Prometeo Program, Coordinación Zonal 5 y 8, Senescyt, Av. Carlos Luis Plaza Da?ín, Edificio del Sector Público del Sector Social, quinto piso, Guayaquil, Ecuador
2.Laboratorio de Biociencias Integradas, Universidad Simon Bolivar, Sartenejas, Baruta, Edo. Miranda - Apartado 89000 Cable Unibolivar, Caracas, Venezuela
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